^ BKKBILL, well I don't really know Bill, I just know they were supplied with every one of 15 or so black tanks we installed around the resort, with instructions to pre-fill the tank with water up to a certain level and add the packet (hopefully 30 days before use). I can only suppose, that was to let the culture establish itself, before the onset of human waste.

Some thing along the lines of HB's sour dough bread starter.