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  1. #1
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    "Astaxanthin" the ultimate anti-aging/antioxidant

    Coming from a Science background, I have always been interested in health and how to enhance it naturally from within.

    I'm trying to minimize my supplements, to a few core elements, which have the most body of Scientific proof to validate their health benefits.

    Astaxanthin is perhaps the very best nutrient for anyone over the age of 40 to take on a daily basis. Although I'm still in my early 30's, I want to build/maintain my health for later years.

    Also, I will sell some key NZ health products in Japan, so I'm experimenting with the best herbs/supps on the market.

    My Nana (95) has macular degeneration disease - and noticed Astaxanthin was a key component of her Ocuvite prescription from her Dr. so thought it would be a good ingredient to help protect my eyes.

    Anyway, I thought I would share it with you guys, so you can perhaps benefit from it as well...

    Here is the skinny on associated benefits of this super-antioxidant:
    • Astaxanthin is believed to be the most potent antioxidant nature has to offer. In terms of antioxidant power or potency, astaxanthin is 550 times stronger than vitamin E, and 6,000 times stronger than vitamin C
    • While it is related to other carotenoids like beta-carotene, lutein, and canthaxanthin, it has a unique structure, and works in some very unique ways. It acts on at least five different inflammation pathways, and can handle far more free radicals at any given time
    • The combination of its high-potency antioxidant and anti-inflammatory properties allows it to address a vast array of health concerns, including joint problems such as rheumatoid arthritis, carpal tunnel syndrome and tennis elbow; protection from UV radiation; improved athletic performance; heart health; brain health, including protection against age-related dementia; and protection against age-related macular degeneration—the most common cause of blindness in the US
    • Astaxanthin Explained

      Astaxanthin is a powerful, naturally occurring carotenoid pigment that's found in certain marine plants and animals. Often called "the king of the carotenoids," astaxanthin is recognized as being one of the most powerful antioxidants found in nature. It is of particular significance, because unlike some other types of antioxidants, astaxanthin never becomes a pro-oxidant in the body so it can never cause harmful oxidation.

      What Are the Benefits of Astaxanthin?


      Astaxanthin is an antioxidant, so it naturally reduces free radicals in the body. But besides that, it also significantly reduces the oxidative load in the body by protecting the cells against oxidation. Because of astaxanthin's unique molecular structure, this red-colored pigment is an extremely powerful antioxidant that is very effective against singlet oxygen. It has a powerful scavenging ability for lipid and free radicals, and effectively breaks peroxide chain reactions.

      While astaxanthin was initially introduced as a "super antioxidant," it has also been found to benefit a number of bodily functions, including:

      Eye Health – The structure of astaxanthin is similar to lutein and zeaxanthin, two antioxidants that have been shown to reduce the risk of nuclear cataracts. But, while similar, astaxanthin has a stronger antioxidant activity and UV-light protection effect, both of which point to it being an excellent supplement for eye health maintenance.

      Skin Health – Astaxanthin has been shown to help improve skin moisture levels, elasticity, and smoothness while reducing wrinkles, freckles, and spots.

      Astaxanthin's antioxidant properties have also been shown to help improve heart heath, cellular health, and the body's immune system.

      Astaxanthin Sources


      Astaxanthin is found in its highest natural concentration in wild Pacific sockeye salmon. It is also found in krill, algae, red trout, shrimp, crab, and lobster. Its vibrant red pigmentation is what helps give these marine animals and plants their intense coloring.


    Studies and research data:

    References:
    • Aoi, W., et al. (2004, July 5). Astaxanthin Limits Exercise-Induced Skeletal and Cardiac Muscle Damage in Mice. Antioxidants & Redox Signaling, 5(1), 139-144. Retrieved from http://online.liebertpub.com/doi/abs...08603321223630
    • Astaxanthin. (2014, September). Retrieved fromhttp://www.med.nyu.edu/content?ChunkIID=160132
    • Bloomer, R. J., et al. (2005, August). Astaxanthin supplementation does not attenuate muscle injury following eccentric exercise in resistance-trained men. International Journal of Sport Nutrition and Exercise Metabolism, 15(4), 401-412. Retrieved fromhttp://www.ncbi.nlm.nih.gov/pubmed/16286671
    • Comhaire, F. H., et al. (2005, September). Combined conventional/antioxidant "Astaxanthin" treatment for male infertility: a double blind, randomized trial. Asian Journal of Andrology, 7(3), 257-262. Retrieved fromhttp://www.ncbi.nlm.nih.gov/pubmed/16110353
    • Hussein, G., et al. (2006, April). Antihypertensive potential and mechanism of action of astaxanthin: III. Antioxidant and histopathological effects in spontaneously hypertensive rats. Biology and Pharmaceutical Bulletin, 29(4), 684-688. Retrieved fromhttp://www.ncbi.nlm.nih.gov/pubmed/16595899
    • Ikeuchi, M., et al. (2006, October). Effects of astaxanthin supplementation on exercise-induced fatigue in mice. Biological and Pharmaceutical Bulletin, 29(10), 2106-2110. Retrieved fromhttp://www.ncbi.nlm.nih.gov/pubmed/17015959
    • Kidd, P. (2011, December). Astaxanthin, cell membrane nutrient with diverse clinical benefits and anti-aging potential. Alternative Medicine Review, 16(4), 355-364. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/22214255
    • Kimble, L., et al. (2013, March 30). Astaxanthin mediates inflammation biomarkers associated with arthritis in human chondrosarcoma cells induced with interleukin-1ß. American Journal of Advances Food Science and Technology, 2. 37-51. Retrieved fromhttp://www.uscip.org/paper/ajafst/AJ...rleukin-1B.pdf
    • MacDermid, J., et al. (2012, March). A blinded placebo-controlled randomized trial on the use of astaxanthin as an adjunct to splinting in the treatment of carpal tunnel syndrome. Hand, 7(1), 1-9. Retrieved fromhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280376/
    • Naguib, Y. (2000, April). Antioxidant activities of astaxanthin and related carotenoids. Journal of Agricultural and Food Chemistry, 48(4), 1150-1154. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10775364
    • Teo, I., et al. (2005, November). Antiproliferation and induction of cell death of Phaffia rhodozyma (Xanthophyllomyces dendrorhous) extract fermented by brewer malt waste on breast cancer cells. International Journal of Molecular Medicine, 16(5), 931-936. Retrieved fromhttp://www.ncbi.nlm.nih.gov/pubmed/16211266
    • Tominaga, K., et al. (2012, March). Cosmetic benefits of astaxanthin on human subjects. Acta Biochimica Polonica 59, 43–47. Retrieved fromhttp://www.actabp.pl/pdf/1_2012/43.pdf
    Last edited by NZdick1983; 22-05-2017 at 04:44 PM.

  2. #2
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    Neverna's Avatar
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    Is it easily available? And how much does it cost (compared to other vitamins like Vitamin C)?

  3. #3
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    No idea mate.. are you in Thailand? I got mine on special for $25 for 60 capsules (I bought 2 bottles)...

    It's quite well known in Japan, as that is where the main body of research was conducted.

    I bought this one (6mg of Astaxanthin among other goodies Q10, vit E, Omega 3 and vit D)...

    Good to take with Vit C... here is a tip for you Nev...

    don't just take the cheap Ascorbic acid (you only get 10% or less absorbed by the body)
    try to get "Lyposperic Vit C" it's encapsulated in oil - much better delivery system (body can absorb over 90%).



    AstaSupreme Natural Astaxanthin Super Antioxidant

  4. #4
    Valve Master Latindancer's Avatar
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    You also need to take Vitamin C which has bioflavinoids (Rutin, Hesperidin) with it, as they all occur together in nature. These help strengthen cell walls (amongst other things).

    Thanks for mentioning this compound, Dick. Though I'm naturally wary of anything so concentrated, I'll look for some more research on it.

  5. #5
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    ^^ Cheers, bro.







    (Green owed )

  6. #6
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    Quote Originally Posted by Latindancer
    Thanks for mentioning this Dick. Though I'm naturally wary of anything so concentrated, I'll look for some more research on it.
    Yup, ask Dr. google 55... heaps of info on it.. no sides (not like Vit a) it's a food like Makuka honey.. can't overdose on it.

    Quote Originally Posted by Neverna
    ^^ Cheers, bro.
    You mustn't call me bro.. I detest that term old chap...

    KIDDING BRO! 55 stay healthy guys...

  7. #7
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    Is Astaxanthin Safe?


    Many people have been exposed to, or have ingested some form of astaxanthin without even realizing it. Astaxanthin is common in marine environments, and plays a significant role in the diets of many animals. For example, the bright pink color and vivid skin of salmon is attributed to the ingestion of Astaxanthin. Another example: the well-known flamingo is born a white bird with gray plumage, but their color will change based on diet. A diet rich in carotenoid algae and sea life such as brine shrimp or prawns (which feed on algae) will offer flamingos vivid pink or bright orange plumage.

    In nature, Astaxanthin results in physical characteristics such as bright and colored pigmentation. In the human body, Astaxanthin serves as a powerful antioxidant that is similar in some ways to the well-known beta-carotene. When addressing the question, “Is Astaxanthin safe?” The answer, in a word, is yes. There are no known side effects, contraindications or issues of toxicity from supplementing this potent antioxidant. Its healthful benefits provide reduced oxidative stress throughout the soft tissues of the body. Like beta-carotene, Astaxanthin is an excellent antioxidant for maintaining optimal eye health, as well as healthy skin, joints, and internal organs.

  8. #8
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    Alternatively, eat a sensible balanced diet and get a bit of exercise..

  9. #9
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    ^ or even better, incorporate supps into an already healthy diet.. and exercise regime..

    although I don't really consider astaxanthin a 'supplement'.. it's more a food source..

    *like Manuka honey, or coconut oil...

    Eating a variety of fresh organic foods—and incorporating supernutrients like astaxanthin—is the best approach to health, along with good sleep, exercise.
    Last edited by NZdick1983; 22-05-2017 at 05:58 PM.

  10. #10
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    Damm it ... I started reading the OP because I thought Dickie had discovered Asterix !
    .

  11. #11
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    55 ^ better than that...

    I used to read those comics as a kid (along with MAD)...

    prolly why I'm so weird...

  12. #12
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    Quote Originally Posted by NZdick1983 View Post
    ^ or even better, incorporate supps into an already healthy diet.. and exercise regime..

    although I don't really consider astaxanthin a 'supplement'.. it's more a food source..

    *like Manuka honey, or coconut oil...

    Eating a variety of fresh organic foods—and incorporating supernutrients like astaxanthin—is the best approach to health, along with good sleep, exercise.
    So, this astaxanthin is a food source.
    And you're recommending eating fresh foods, good sleep, exercise etc .

    Is there really any reason to consume this additional so called"super nutrient" other than to make the moronic public believe that the likes of you are doing them good.

    It's bull shit mate and you know it.

    Still, if you can con the great unwashed into believing you and you get to make a few bucks good for you.
    Hard luck on them for being so gullible.
    No disrespect meant.

  13. #13
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    ^ Ay? I'm not selling it (yet) and when/if I do, it will be in Japan. Not to crusty old, cynical farts on this board.

    Not trying to con anyone bud.

    Just taken the time out of my day, to pass on some health food tips for the benefit of people interested.

    I've already listed the numerous benefits of this antioxidant... next you will try to poo poo Manuka honey... lol

    Plenty of information online/studies/clinical research, etc... I'm sure anyone interested will do their own research/due diligence.

    Stick to your crackers and cheeseburgers, no skin off my nose, as you were, healthy man... cheers.


    ----------------

    some more benefits:

    • Reduces proliferation of breast cancer tumor cells by 40%
    • Protects the brain from dementia and Alzheimer's
    • Greatly reduces inflammation and joint pain
    • Reduces oxidative damage to your DNA by 40% (even at low doses)
    • Greatly increases endurance, muscle recovery and workout performance
    • Reduces the risk of cancer
    • Reduces blood sugar level in diabetics and prediabetics
    • Improves fertility while decreasing the rate of stillborn births
    • Promotes cardiovascular health, reduces C-Reactive Proteins (CRP)
    • Reduces or eliminates carpal tunnel syndrome
    • Boosts immune function and helps the body resist infections
    • Protects the stomach from ulcers and invasive bacteria
    • Protects the kidneys from damage due to high blood sugar
    • Causes cancer cells to commit suicide (apoptosis)
    • Greatly improves sperm quality, motility, and sperm count
    • Prevents asthma by normalizing histamine levels
    • Protects the body from highly oxidative foods like fried foods

    In addition, I've found that this natural medicine supplement:

    • Greatly protects eye health, reduces cataracts and prevents UV damage to the eyes

    • Makes skin look younger and functions as a natural internal sunscreen that prevents DNA damage and sunburn

    • Protects the body from the dangerous oxidizing effects of Vioxx and COX-2 inhibitors, meaning that is can actually reduce the harmful side effects and deaths caused by other anti-inflammatory pharmaceuticals
    Last edited by NZdick1983; 22-05-2017 at 06:57 PM.

  14. #14
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    Aging is part of the natural order of things - it just is.

  15. #15
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    Quote Originally Posted by NZdick1983 View Post
    ^ Ay? I'm not selling it (yet) and when/if I do, it will be in Japan. Not to crusty old, cynical farts on this board.

    Not trying to con anyone bud.

    Just taken the time out of my day, to pass on some health food tips for the benefit of people interested.

    I've already listed the numerous benefits of this antioxidant... next you will try to poo poo Manuka honey... lol

    Plenty of information online/studies/clinical research, etc... I'm sure anyone interested will do their own research/due diligence.

    Stick to your crackers and cheeseburgers, no skin off my nose, as you were, healthy man... cheers.


    <hr><hr><hr>-

    some more benefits:

    • Reduces proliferation of breast cancer tumor cells by 40%
    • Protects the brain from dementia and Alzheimer's
    • Greatly reduces inflammation and joint pain
    • Reduces oxidative damage to your DNA by 40% (even at low doses)
    • Greatly increases endurance, muscle recovery and workout performance
    • Reduces the risk of cancer
    • Reduces blood sugar level in diabetics and prediabetics
    • Improves fertility while decreasing the rate of stillborn births
    • Promotes cardiovascular health, reduces C-Reactive Proteins (CRP)
    • Reduces or eliminates carpal tunnel syndrome
    • Boosts immune function and helps the body resist infections
    • Protects the stomach from ulcers and invasive bacteria
    • Protects the kidneys from damage due to high blood sugar
    • Causes cancer cells to commit suicide (apoptosis)
    • Greatly improves sperm quality, motility, and sperm count
    • Prevents asthma by normalizing histamine levels
    • Protects the body from highly oxidative foods like fried foods

    In addition, I've found that this natural medicine supplement:

    • Greatly protects eye health, reduces cataracts and prevents UV damage to the eyes

    • Makes skin look younger and functions as a natural internal sunscreen that prevents DNA damage and sunburn

    • Protects the body from the dangerous oxidizing effects of Vioxx and COX-2 inhibitors, meaning that is can actually reduce the harmful side effects and deaths caused by other anti-inflammatory pharmaceuticals
    Good.
    Show us the clinical trials that have proved your claims.
    Please.

  16. #16
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    Quote Originally Posted by NZdick1983
    ^ Ay? I'm not selling it (yet) and when/if I do, it will be in Japan. Not to crusty old, cynical farts on this board.
    Oh, so selling it to Japanese folk who are not questioning or cynical is ok is it.
    I remember working in Darfur years ago when folk would try to sell baby milk powder to mothers. It was, of course, shit and kids died. The publicity campaign was good though and it kept on selling for quite a while.

    I'm not putting you down Dick but I'd like to see the evidence that proves what you say about this stuff.

  17. #17
    Member Thedogsbollix's Avatar
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    Maybe Elvis should bunged some of that shit in his Cheese burgers.

    The Fat coont may still be alive singing Blue swade shoes.

    It's all bulls hit NZ nigger, every sick fuk would be on it if it really worked.

    Anyway, sell it to the Dolphin eaters, those retards would by anything including Australia.

  18. #18
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    Quote Originally Posted by crackerjack101
    Good. Show us the clinical trials that have proved your claims. Please.
    Altern Med Rev. 2011 Dec;16(4):355-64.
    Astaxanthin, cell membrane nutrient with diverse clinical benefits and anti-aging potential.

    Kidd P1.
    Author information


    Abstract

    Astaxanthin, a xanthophyll carotenoid, is a nutrient with unique cell membrane actions and diverse clinical benefits. This molecule neutralizes free radicals or other oxidants by either accepting or donating electrons, and without being destroyed or becoming a pro-oxidant in the process. Its linear, polar-nonpolar-polar molecular layout equips it to precisely insert into the membrane and span its entire width. In this position, astaxanthin can intercept reactive molecular species within the membrane's hydrophobic interior and along its hydrophilic boundaries. Clinically, astaxanthin has shown diverse benefits, with excellent safety and tolerability. In double-blind, randomized controlled trials (RCTs), astaxanthin lowered oxidative stress in overweight and obese subjects and in smokers. It blocked oxidative DNA damage, lowered C-reactive protein (CRP) and other inflammation biomarkers, and boosted immunity in the tuberculin skin test. Astaxanthin lowered triglycerides and raised HDL-cholesterol in another trial and improved blood flow in an experimental microcirculation model. It improved cognition in a small clinical trial and boosted proliferation and differentiation of cultured nerve stem cells. In several Japanese RCTs, astaxanthin improved visual acuity and eye accommodation. It improved reproductive performance in men and reflux symptoms in H. pylori patients. In preliminary trials it showed promise for sports performance (soccer). In cultured cells, astaxanthin protected the mitochondria against endogenous oxygen radicals, conserved their redox (antioxidant) capacity, and enhanced their energy production efficiency. The concentrations used in these cells would be attainable in humans by modest dietary intakes. Astaxanthin's clinical success extends beyond protection against oxidative stress and inflammation, to demonstrable promise for slowing age-related functional decline.

  19. #19
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    too many to list...

    Mar Drugs. 2011; 9(3): 447–465.
    Published online 2011 Mar 21. doi: 10.3390/md9030447


    PMCID: PMC3083660




    Astaxanthin: A Potential Therapeutic Agent in Cardiovascular Disease

    Robert G. Fassett1,2,* and Jeff S. Coombes2

    Author information ► Article notes ► Copyright and License information ►

    This article has been cited by other articles in PMC.



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    Abstract

    Astaxanthin is a xanthophyll carotenoid present in microalgae, fungi, complex plants, seafood, flamingos and quail. It is an antioxidant with anti-inflammatory properties and as such has potential as a therapeutic agent in atherosclerotic cardiovascular disease. Synthetic forms of astaxanthin have been manufactured. The safety, bioavailability and effects of astaxanthin on oxidative stress and inflammation that have relevance to the pathophysiology of atherosclerotic cardiovascular disease, have been assessed in a small number of clinical studies. No adverse events have been reported and there is evidence of a reduction in biomarkers of oxidative stress and inflammation with astaxanthin administration. Experimental studies in several species using an ischaemia-reperfusion myocardial model demonstrated that astaxanthin protects the myocardium when administered both orally or intravenously prior to the induction of the ischaemic event. At this stage we do not know whether astaxanthin is of benefit when administered after a cardiovascular event and no clinical cardiovascular studies in humans have been completed and/or reported. Cardiovascular clinical trials are warranted based on the physicochemical and antioxidant properties, the safety profile and preliminary experimental cardiovascular studies of astaxanthin.

    Keywords: antioxidants, xanthophyll carotenoid, inflammation, Haematococcus pluvialis, oxidative stress

    Go to:
    1. Introduction

    Astaxanthin is a xanthophyll carotenoid of predominantly marine origin, with potent antioxidant and anti-inflammatory effects demonstrated in both experimental and human studies. Oxidative stress and inflammation are common pathophysiological features of atherosclerotic cardiovascular disease hence astaxanthin may have a potential therapeutic role in this condition. This review will summarise the available evidence suggesting astaxanthin may be of therapeutic value in cardiovascular disease.

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    2. Oxidative Stress and Inflammation

    Oxidative stress and inflammation are established non-traditional risk factors for atherosclerosis associated cardiovascular morbidity and mortality [1]. Dietary antioxidants can reduce the oxidation of lipids and proteins and have the potential to protect from the development of arterial stiffening and atherosclerosis [24]. Cross-sectional and prospective observational studies have demonstrated an association between the intake of dietary antioxidants and/or their plasma levels and a reduction of cardiovascular events [510]. This supports the theory that oxidative stress is a pathophysiological process involved in atherosclerotic vascular damage. Also, a reduced dietary antioxidant intake is associated with oxidative stress and inflammation [11]. Newer more potent dietary antioxidants such as astaxanthin have yet to be studied in this setting. Studies that have assessed the intake of β-carotene or dietary β-carotene supplementation have shown higher β-carotene consumption is associated with a reduction in cardiovascular disease [6,1217]. Other than a few studies [1820], cardiovascular intervention trials using antioxidants have not demonstrated benefits [2123]. This may be because study participants did not have oxidative stress and/or the antioxidants used were insufficiently potent. In addition, it is becoming recognized that there is communication between oxidative stress and inflammatory processes leading to the additional hypothesis that antioxidants may be able to modify both deleterious events. Further research is needed studying antioxidants with different biological actions in patients with demonstrated oxidative stress.

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    3. Carotenoids

    Carotenoids are ubiquitous, and found in high concentrations in plants, algae and microorganisms. Humans and other animals cannot synthesize them and therefore are required to source them in their diet [24]. Carotenoids are classified, according to their chemical structure, into carotenes and xanthophylls. The carotene carotenoids include β-carotene and lycopene and the xanthophyll carotenoids include lutein, canthaxanthin, zeaxanthin, violaxanthin, capsorubin and astaxanthin [25,26].
    The effects of carotenoids vary dependent on how they interact with cell membranes [25]. The effects of astaxanthin, zeaxanthin, lutein, β-carotene and lycopene on lipid peroxidation have been assessed using a polyunsaturated fatty acid enriched membrane model [25,27]. Non-polar carotene carotenoids such as lycopene and β-carotene caused membrane disorder and lipid peroxidation in contrast to the polar xanthophyll carotenoid astaxanthin, which preserved membrane structure [27]. Contrasting effects of different carotenoids may be responsible for the differing biological effects seen in clinical studies. For instance, in some studies the non-polar carotenoid, β-carotene has been shown to have no benefit on cardiovascular disease [2832] and in fact it may be pro-oxidant at high doses [33]. In contrast, the polar carotenoid astaxanthin has protective effects on the cardiovascular system demonstrated in animal studies. However, this has not been studied in human clinical trials [3436]. β-carotene at physiological levels may act in differing ways when ultraviolet A light A (UVA) acts on keratinocytes including vitamin A-independent pathways [37]. Astaxanthin, canthaxanthin and β-carotene had differential effects on UVA-induced oxidative damage [38]. In addition, carotenoids may also alter the immune response [39] and transcription [40].

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    4. Astaxanthin

    Astaxanthin contains two oxygenated groups on each ring structure (see Figure 1), which is responsible for its enhanced antioxidant features [41]. It is found in living organisms particularly in the marine environment where it is present in microalgae, plankton, krill and seafood. It gives salmon, trout, and crustaceans such as shrimp and lobster their distinctive reddish coloration [42]. It is also present in yeast, fungi, complex plants and the feathers of some birds including flamingos and quail [42]. In 1987, the United States Food and Drug Administration approved astaxanthin as a feed additive for use in the aquaculture industry and in 1999 it was approved for use as a dietary supplement (nutraceutical) [41]. The microalgae Haematococcus pluvialis produces the astaxanthin isomer (3S, 3S′), which is the same as the form found in wild salmon. Synthesis of astaxanthin is not possible in humans and it cannot be converted to vitamin A, which means excess intake will not cause hypervitaminosis A toxicity [43,44]. Astaxanthin and canthaxanthin are scavengers of free radicals, chain-breaking antioxidants and potent quenchers of reactive oxygen and nitrogen species including singlet oxygen, single and two electron oxidants [4547]. They (astaxanthin and canthaxanthin) have terminal carbonyl groups that are conjugated to a polyene backbone [26] and are more potent antioxidants and scavengers of free radicals than carotene carotenoids such as β-carotene [47,48]. For these reasons dietary supplementation with astaxanthin has the potential to provide antioxidant protection of cells and from atherosclerotic cardiovascular disease [49].
    Figure 1.
    Molecular structure of astaxanthin.



    Go to:
    5. Astaxanthin Formulations

    5.1. Astaxanthin of Marine Origin

    Astaxanthin used in nutritional supplements is usually a mixture of configurational isomers produced by Haematococcus pluvialis, a unicellular microalga [50]. Astaxanthin can be produced in its natural forms on a large scale [51]. The initial production of astaxanthin from Haematococcus pluvialis uses closed culture systems followed by a 5–7 day, “reddening” cycle, conducted in open culture ponds. At each production stage, the cultures are closely monitored by microscopic examination to ensure they remain free of contamination. After the reddening cycle, Haematococcus pluvialis cultures are harvested, washed and dried. The final step for the production of astaxanthin is extraction of dried Haematococcus pluvialis biomass using supercritical carbon dioxide to produce a purified oleoresin, which is free of any contamination. Other sources used for the commercial production of astaxanthin include cultures of Euphausia pacifica (Pacific krill), Euphausia superba (Antarctic krill), Pandalus borealis (shrimp) and Xanthophyllomyces dendrorhous, formerly Phaffia rhodozyma (yeast). Astaxanthin from natural sources varies considerably from one organism to another. For instance, the astaxanthin found in seafood will depend on the stereoisomer ingested. Astaxanthin produced by haematococcus pluvialis, consists of the (3-S,3′-S) stereoisomer which is most commonly used in aquaculture. It is therefore the form most commonly consumed by humans.

    5.2. Synthetic Astaxanthin

    There are three stereoisomers of astaxanthin; (3-R,3′-R), (3-R,3′-S) and (3-S,3′-S). Disodium disuccinate astaxanthin (DDA) is a synthetic astaxanthin containing a mixture of all three stereoisomers, in the proportions 1:2:1. DDA was manufactured by Cardax Pharmaceuticals and used in animal studies investigating the myocardial ischemia-reperfusion injury models [3436,5254]. This form of astaxanthin was touted to have better aqueous solubility, unlike other carotenoids, and this enabled both oral and intravenous administration. DDA is no longer available but the same company now produces a second synthetic astaxanthin compound; Heptax/XanCor, CDX-085. The company claims that it is developed for thrombotic protection, triglyceride reduction, metabolic syndrome, and inflammatory liver disease. In addition, it has increased water dispersibility and enhanced bioavailability compared to natural astaxanthin and DDA. The synthetic forms are metabolized via hydrolysis in the intestine yielding free astaxanthin for intestinal absorption. CDX-085 has been used in one study, discussed below [55].
    It is not yet clear which form of astaxanthin should be administered in clinical studies, the natural form from the marine environment or a synthetic form. As the proportions of stereoisomers, vary between these different forms of astaxanthin they may not be therapeutically equivalent [56]. Thus synthetic astaxanthin could result in different outcomes when assessed clinically [57].


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    6. Astaxanthin-Experimental Studies

    Experimental studies undertaken with astaxanthin specifically relevant to the cardiovascular system are summarised in Table 1. Astaxanthin attenuates mediators of oxidative stress and inflammation and has shown beneficial effects in non-cardiovascular models of disease [5869]. In addition, astaxanthin has decreased markers of lipid peroxidation [70], inflammation [61,62,67,68] and thrombosis [55].
    Table 1.
    Animal studies investigating the cardiovascular effects of astaxanthin.


    6.1. Cardiovascular Studies

    A series of experiments have been conducted to assess the efficacy of DDA in protecting the myocardium using the myocardial ischemia-reperfusion model in rats, rabbits and dogs [35,36,53,54]. Prior treatment for four-days with intravenous DDA using doses of 25, 50 and 75 mg/kg body weight in Sprague-Dawley rats significantly reduced myocardial infarct size [35]. The degree of cardiac protection correlated with the dose of DDA administered. In a study in rabbits using a myocardial ischaemia-reperfusion model prior intravenous treatment with 50 mg/kg/day of DDA for four days resulted in a significant decrease in the size of the myocardial infarction and an improvement in myocardial salvage [52]. Animals treated with DDA had an attenuation of inflammation and complement activation suggesting there was a reduction in tissue inflammation [52]. In another study using a dog model intravenous DDA was administered daily for four-days prior to occlusion of the left anterior descending coronary artery or two hours prior to coronary artery occlusion [54]. After an hour of coronary occlusion and three hours of reperfusion there was a significant reduction in myocardial infarct size in the dogs treated with DDA. In the four-day treatment group, two out of three dogs had complete cardiac protection [54]. In a rat study, the effects of seven days of pre-treatment with oral DDA, 125 and 500 mg/kg/day on the concentrations of free astaxanthin in myocardial tissue [36]. The astaxanthin concentration in the myocardium was 400 nM after oral DDA at a dose of 125 mg/kg/day for seven-days and it was 1634 nM after 500 mg/kg/day. There was also a reduction of multiple lipid peroxidation products. The doses of DDA used in these experiments were quite high and at this stage it is not known whether such doses would be safe to use in humans.
    The effects of astaxanthin on blood pressure (BP) were assessed in spontaneously hypertensive rats (SHR). There was a significant reduction in BP after 14-days of oral astaxanthin administration whereas this did not occur in normotensive Wistar Kyoto rats [71]. Astaxanthin administered orally for five-weeks in stroke prone SHR also resulted in a significant BP reduction [71]. Oral astaxanthin also enhanced nitric oxide induced vascular relaxation in the rat aortas [71] In experiments in SHR, oral astaxanthin significantly decreased nitric oxide end products indicating that it may be exerting its BP effects via this pathway [72]. Studies using the SHR aorta and coronary arteries demonstrated that astaxanthin reduced the wall/lumen ratio in coronary arteries and decreased elastin bands in the aorta [72]. This suggests that astaxanthin may beneficially mediate atherosclerotic CVD processes.
    Recently, a series of two experiments were reported in the one article, one using the synthetic astaxanthin (CDX-085) and the other using free astaxanthin [55]. CDX-085 administered orally to C57BL/6 mice resulted in the presence of free astaxanthin in the plasma, heart, liver and platelets. Mice that were fed astaxanthin had significantly increased basal arterial blood flow and a delay in occlusive thrombosis after endothelial injury. Also, in an in vitro study, human umbilical vein endothelial cells and platelets isolated from Wistar-Kyoto rats that were treated with free astaxanthin has significantly increased nitric oxide release and a decrease in peroxynitrite levels [55]. The authors concluded the results support the potential of astaxanthin as a potential therapy to prevent thrombosis associated with cardiovascular disease.
    Astaxanthin administered to C57BL/6 mice resulted in a reduction in exercise-induced increases in the oxidative stress biomarkers 8-hydroxy-2′-deoxyguanosine and 4-hydroxy-2-nonenal-modified protein in both cardiac and gastrocnemius muscle [63]. Increases in myeloperoxidase and creatinine kinase activity in cardiac and gastrocnemius muscle were also reduced by astaxanthin. After three-weeks of astaxanthin supplementation there was evidence of accumulation of astaxanthin in gastrocnemius and cardiac muscle. Astaxanthin given to female BALB/c mice for eight-weeks resulted in a dose dependent increase in plasma astaxanthin but no significant changes in blood glutathione or change in lymphocyte mitochondrial membrane potential and cardiac contractility index measured on echocardiography. The mice that were fed 0.08% astaxanthin in the diet had higher cardiac mitochondrial membrane potential and contractility index compared with control animals [74]. This suggests dietary astaxanthin provides cardiac protection. Astaxanthin administered for four weeks to eight week old ICR mice resulted in increased exercised induced fat utilization and prevention of increased hexanoyl-lysine modification of carnitine palmitoyltransferase I (CTP I) [73]. In a canine carotid artery thrombosis model, administration of DDA resulted in a dose-dependent reduction in carotid artery re-thrombosis and a reduction of re-thrombosis after thrombolysis but there was no effect on hemostasis [34].

    6.2. Diabetes Studies

    Diabetes mellitus and its associated nephropathy is a common cause of chronic kidney disease and is complicated by accelerated atherosclerotic cardiovascular disease [75]. In studies involving diabetic db/db mice, supplementation with astaxanthin produced a reduction in the levels of blood glucose [60]. In the kidney there was significantly decreased relative mesangial area in the animals supplemented with astaxanthin. Also proteinuria and urinary excretion of 8-OHdG were attenuated. Mice supplemented with astaxanthin had less glomerular 8-OHdG immunoreactive cells [60]. Hyperglycemia induced reactive oxygen species production, activation of transcription factors, and cytokine expression and production by normal human mesangial cells was suppressed significantly by astaxanthin [66].


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    7. Astaxanthin Studies in Humans

    Although no cardiovascular outcomes studies using astaxanthin have been reported in humans there have been clinical studies that have investigated the effects of astaxanthin in human health and other diseases (Table 2). The majority of these have been conducted in healthy participants who volunteered to assess astaxanthin dose, bioavailability, safety and oxidative stress, which are all potentially relevant to the cardiovascular system. Studies have also been conducted in other medical conditions such as reflux oesophagitis, where measurements of oxidative stress and/or inflammation have been included.
    Table 2.
    Clinical studies investigating the safety, bioavailability and effects of astaxanthin on oxidative stress.


    7.1. Dosing

    Human clinical studies have used oral astaxanthin in a dose that ranges from 4 mg up to 100 mg/day, given from a one off dose up to durations of one-year (Table 2).

    7.2. Bioavailability

    Astaxanthin bioavailability from the marine environment was assessed in a randomised double blind trial in 28 volunteers [83]. Participants were given either 250 g of wild salmon or aquaculture salmon (5 μg/g) to eat. Wild salmon ingest astaxanthin naturally from krill whereas aquacultured salmon acquire it from fish that are fed astaxanthin that might be derived from a synthetic source. Plasma levels of astaxanthin were higher at 3, 6, 10 and 14 days during ingestion of the aquacultured compared with the wild salmon. Plasma levels of the (3-S, 3′-S) isomer of astaxanthin appeared at higher levels than its proportionate level in the flesh of the salmon. This suggests that isomers of astaxanthin might have different bioavailability. The plasma isomers of astaxanthin have also been studied after ingestion of single oral dose of 10mg and also 100 mg over four-weeks. Astaxanthin plasma elimination half-life was 52 (SD 40) h and there was a non-linear dose response and selective absorption of z-isomers [79].

    7.3. Safety

    The safety of astaxanthin administered orally was assessed in a double-blind, randomised placebo-controlled trial undertaken in healthy adults [78]. Volunteers took either 6 mg/day of astaxanthin or placebo for eight-weeks. BP and biochemistry measured after four and eight weeks of therapy revealed no significant differences in these parameters between treatment and placebo groups and these did not differ from baseline. The authors concluded that healthy adults could safely consume 6 mg/day of astaxanthin derived from a Haematococcus pluvialis algal extract. Measuring whole blood transit time in 20 healthy males was used to assess the effects of astaxanthin on blood rheology in humans. Six milligrams of oral astaxanthin per day for ten days improved blood rheology as evidenced by decreased whole blood transit time [82]. Escalating concentrations of astaxanthin were tested in vitro with blood taken from volunteers, 8 of whom were taking asprin and 12 who were not [85]. Even supra-therapeutic concentrations of astaxanthin had no adverse effects on indices of platelet, coagulation and fibrinolytic function. These results support the safety profile of astaxanthin for future clinical trials. No significant side effects have been reported so far in published human studies in which astaxanthin was administered to humans.

    7.4. Oxidative Stress and Inflammation

    Oral supplementation with astaxanthin in studies in healthy human volunteers and patients with reflux oesophagitis demonstrated a significant reduction in oxidative stress, hyperlipidemia and biomarkers of inflammation [70,80,86]. In a study involving 24 healthy volunteers who ingested astaxanthin in doses from 1.8 to 21.6 mg/day for two weeks, LDL lag time, as a measure of susceptibility of LDL to oxidation, was significantly greater in astaxanthin treated participants indicating inhibition of the oxidation of LDL [70]. Plasma levels of 12- and 15-hydroxy fatty acids were significantly reduced in 40 healthy non-smoking Finnish males given astaxanthin [80] suggesting astaxanthin decreased the oxidation of fatty acids [80]. The effects of dietary astaxanthin in doses of 0, 2 or 8 mg/day, over 8 weeks, on oxidative stress and inflammation were investigated in a double blind study in 14 healthy females [84]. Although these participants did not have oxidative stress or inflammation those taking 2 mg/day had lower CRP at week eight. There was also a decrease in DNA damage measured using plasma 8-hydroxy-2′-deoxyguanosine after week four in those taking astaxanthin. Astaxanthin therefore appears safe, bioavailable when given orally and is suitable for further investigation in humans.


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    8. Clinical Trial Using Astaxanthin

    A double-blind randomised placebo-controlled clinical trial (Xanthin study) is currently being conducted to assess the effects of astaxanthin 8mg orally day on oxidative stress, inflammation and vascular function in patients that have received a kidney transplant [87]. Patients in the study undertake measurements of surrogate markers of cardiovascular disease including aortic pulse wave velocity, augmentation index, brachial forearm reactivity and carotid artery intima-media thickness. Depending on the results from this pilot study a large randomised controlled trial assessing major cardiovascular outcomes such as myocardial infarction and death may be warranted.

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    9. Conclusions

    Experimental evidence suggests astaxanthin may have protective effects on cardiovascular disease when administered prior to an induced ischemia-reperfusion event. In addition, there is evidence that astaxanthin may decrease oxidative stress and inflammation which are known accompaniments of cardiovascular disease. At this stage we do not know whether astaxanthin has any therapeutic value in human cardiovascular disease either in a preventative capacity or when administered after a cardiovascular insult. It has been proposed that astaxanthin may provide cardiovascular protection through reducing oxidative stress, which is one of the non-traditional risk factors for the development of atherosclerotic cardiovascular disease. The role of oxidative stress in cardiovascular disease is supported by evidence from observational studies that have found associations between antioxidant intake, oxidative stress and cardiovascular outcomes. Despite this, clinical intervention studies using antioxidants including vitamin E, β-carotene and vitamin C, have not proved successful [22,23]. These intervention studies may have failed because of flawed design where patients were not included based on the presence of oxidative stress. Hence, many participants may not have been in a state of oxidative stress and able to benefit from antioxidant therapy. Also, in those participants where oxidative stress may have existed there was no way of assessing whether the therapy adequately corrected this. Thus, the antioxidants used such as vitamin E, β-carotene and vitamin C may not have been effective because insufficient doses were used or an inadequate length of therapy followed to correct the oxidative stress. Some antioxidants such as β-carotene may be pro-oxidant at higher doses, which could have confounded study results.
    Astaxanthin is a potent antioxidant and based on its physicochemical properties and the results of preliminary experimental studies in ischaemia-reperfusion models of cardiovascular disease, it warrants consideration for testing in human clinical trials. There have been no safety concerns noted so far in human clinical studies where astaxanthin has been administered. As astaxanthin is a potent antioxidant and is associated with membrane preservation, it may protect against oxidative stress and inflammation and provide cardiovascular benefits.

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    Acknowledgments

    Cyanotech the manufacturer of BioAstin, a proprietary brand of astaxanthin, is providing financial support and astaxanthin capsules and placebo for a clinical trial being conducted by the authors. The sponsor has no role in the study itself.


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    Footnotes

    Samples Availability: Available from the authors.


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  20. #20
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    Quote Originally Posted by crackerjack101
    Oh, so selling it to Japanese folk who are not questioning or cynical is ok is it.
    The Japanese are affluent consumers who set trends in. Asia. The attitudes .... expect a highly-educated consumer base with significant disposable income and a desire to increase consumption of sophisticated and niche health benefits.

    Japanese are very intelligent consumers, hence their quick uptake of Manuka honey (UMF) and other key health foods.

    Quote Originally Posted by Thedogsbollix
    Anyway, sell it to the Dolphin eaters, those retards would by anything including Australia.
    Those kangaroo eating convicts... yeah.. educated aussie consumers lol...

    Japanese study...

    New study shows astaxanthin is effective against daily mental and physical fatigue
    By Gary Scattergood+, 07-Nov-2016
    Japanese nutraceutical firm AstaReal says its astaxanthin has been found to be simultaneously effective against both mental and physical fatigue in a new published study.

    http://www.nutraingredients-asia.com/Research/New-study-shows-astaxanthin-is-effective-against-daily-mental-and-physical-fatigue

    ..and if you don't believe the antioxidant effects, even given hundreds of clinical trials... well... can't argue with it's ability to protect the eyes from Macular degeneration..
    as it's prescribed by hospitals and doctors for that specifically.. so if you want to keep your eye sight sharp... or stop Macular degeneration... might want to re-consider na krub.

    up to you...

    Last edited by NZdick1983; 22-05-2017 at 07:53 PM.

  21. #21
    Member Thedogsbollix's Avatar
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    Give Trump a call, he will buy all ya stock.

    He needs it concidering he will be top dog for the next 3 terms.

    Sell him some of ya whitenning cream as well, he needs to drop the orange glow.

    It's scaring the Saudis at the moment.

  22. #22
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    ^ No stock yet (just the couple bottles I bought today)... and it's for my own use...

    Nah... my bro, 'The Rock' will be the next US President...

    ...I don't have enough whitening cream to bring my nigga to honkey.... heh

  23. #23
    Member Thedogsbollix's Avatar
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    ^
    Rub it on ya cock and let us know if it loosens up ya wrinkles.

    Ya would make a Killin selling it to the crusty old coonts on this forum.

  24. #24
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    One can only be so healthy.

    Beyond that, becomes delusional and obsessive.

  25. #25
    Member Thedogsbollix's Avatar
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    Dumb people would by anything if ya tell them they will live longer.

    Unfortunately it has never worked.

    Best just to save the money for a proper cause, that being Sex, Drugs and Rock and Roll.

    In no particular order of course.

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